Data/hora: 17/06/2024 12:52:00

Provedor de dados: | 55
País: | Brazil
Título: | Characteristic profile of antibody responses to PPD, ESAT-6, and CFP-10 of Mycobacterium tuberculosis in pulmonary tuberculosis suspected cases in Surabaya, Indonesia
Autores: | Dewi,Desak Nyoman Surya Suameitria; Mertaniasih,Ni Made; Soedarsono,; Ozeki,Yuriko; Artama,Wayan Tunas; Fihiruddin,; Niki,Mamiko; Tateishi,Yoshitaka; Ato,Manabu; Matsumoto,Sohkichi.
Data: | 2019-08-01
Ano: | 2019
Palavras-chave: | Antibody response' profile; PPD; Pulmonary tuberculosis; NTM.
Resumo: | Abstract Accurate and rapid diagnostic tools are important aspects of managing tuberculosis (TB) cases appropriately. However, the sensitivity and specificity of diagnostic kits based on immune response such as the tuberculin skin test (TST) and interferon gamma release assay (IGRA) are still debated. Thus, the exploration and assessment of specific biomarker-targeted antibodies are needed for the development of an accurate and rapid diagnostic tool. The present study was conducted in patients with a respiratory problem suspected to be TB at Dr. Soetomo Hospital, Surabaya, Indonesia. Among 102 patients tested by GeneXpert and AFB, 59 serum samples were from cases retrospectively determined to have active TB. A total of 102 serum of healthy controls (HC) was also collected. The PPD antigen and the recombinant CFP-10 and ESAT-6 proteins were prepared. Antibody responses against these proteins were evaluated by ELISA. All samples were also screened for the possibility of Mycobacterium avium-intracellulare complex (MAC) infection using Capilla MaC kit. The results showed that TB patients had a significantly higher concentration of IgG antibody in response to PPD than the HC. In addition, the receiver operating characteristic (ROC) curve analysis showed that PPD was acceptable for diagnostic purposes with an AUC value of 0.835 (95% CI 0.770-0.900, p &lt; 0.0001). However, ESAT-6 and CFP-10 had low AUCs, and 32 samples from both groups showed a low concentration of IgA antibody against all antigens. The MAC detection results also showed that the concentration of IgA in the HC group was the highest. The current results indicate that PPD is a better antigen for antibody-based detection of TB than ESAT-6 and CFP-10. Based on the MAC detection assay, 53 people in the HC group were probably infected with rapidly growing nontuberculous mycobacteria (NTM), although antibody response to PPD was low.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000400246
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2019.07.001
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.23 n.4 2019
Fonte: | info:eu-repo/semantics/openAccess