Data/hora: 09/06/2024 09:22:59

Provedor de dados: | Brazilian Journal of Infectious Diseases
País: | Brazil
Título: | The application of latent class analysis for diagnostic test validation of chronic Trypanosoma cruzi infection in blood donors
Autores: | Langhi Junior,Dante M.; Bordin,José O.; Castelo,Adauto; Walter,Stephen D.; Moraes-Souza,Hélio; Stumpf,Robert J.
Data: | 2002-08-01
Ano: | 2002
Palavras-chave: | Chaga's disease; Blood donors; Diagnostic test; Latent class analysis.
Resumo: | The main strategy to prevent transfusion-associated Chagas' disease is the identification of T. Cruzi-infected blood donors by serological screening tests, however there is no perfect serological gold standard. We evaluated an enzyme immunoassay (EIA), an indirect hemaglutination (IHA), and an indirect immunofluorescence (IIF) test for detecting T. Cruzi antibodies in Brazilian blood donors. The results were submitted to latent class analysis, and a radioimmunopreciptation (RIPA) test was performed on repeatedly positive samples. Among 1951 donors, 11 (0.56%) were positive by EIA, 6 (0.31%) by IHA and 16 (0.82%) by IIF. Six samples were positive with all tests, while 4 reacted with EIA and IIF. The RIPA was positive in 6 (75.0%), 7 (66.6%), and 4 (54.0%) samples reacting by the EIA, IHA and IIF tests, respectively. The latent class model detected a high sensitivity rate (100%) for the EIA and IIF, and a specificity rate of 99.95% and 99.69% for the EIA and IIF tests, respectively. The probability of being case according to the model was 99.92% when both EIA and IIF were positive, and 100% for the association of EIA, IIF, and IHA.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000400005
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702002000400005
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.6 n.4 2002
Fonte: | info:eu-repo/semantics/openAccess