Data/hora: 11/06/2024 04:44:38

Provedor de dados: | 55
País: | Brazil
Título: | Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
Autores: | de-Paris,Fernanda; Machado,Alice Beatriz Mombach Pinheiro; Gheno,Tailise Conte; Ascoli,Bruna Maria; Oliveira,Kátia Ruschel Pilger de; Barth,Afonso Luis.
Data: | 2011-08-01
Ano: | 2011
Palavras-chave: | Streptococcus agalactiae; Polymerase chain reaction; Culture; Pregnancy.
Resumo: | Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702011000400004
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.15 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess