Data/hora: 01/06/2024 16:22:27

Provedor de dados: | Brazilian Journal of Infectious Diseases
País: | Brazil
Título: | Predominance of Leishmania major and rare occurrence of Leishmania tropica with haplotype variability at the center of Iran
Autores: | Zahirnia,Amir Hossein; Bordbar,Ali; Ebrahimi,Sahar; Spotin,Adel; Mohammadi,Somayeh; Ghafari,Seyedeh Maryam; Ahmadvand,Setareh; Jabbari,Negar; Esmaeili Rastaghi,Ahmad Reza; Parvizi,Parviz.
Data: | 2018-08-01
Ano: | 2018
Palavras-chave: | Leishmaniasis; Leishmania major; Leishmania tropica; Nuclear gene; Iran.
Resumo: | ABSTRACT  Background Leishmania major is a causative agent of zoonotic cutaneous leishmaniasis in the center of Iran, Abarkouh district. Molecular characterization and precise incrimination of Leishmania species was carried out to perform controlling measurements and to design treatment programs for zoonotic cutaneous leishmaniasis.  Methods All smears isolated from ulcers of suspected patients were examined under a light microscope and graded for amastigotes frequency. Extraction of DNA, PCR, RFLP and sequencing of ITS-rDNA genotype were done to increase the efficacy of Leishmania parasites identification at their species-specific level and to detect any Leishmania infections within.  Results Humans were found to be infected with L. major with high infection frequency and also Leishmania tropica was identified with low occurrence for the first time as non-native species using molecular analyses. The rates of infections was considerable with microscopic observation (n= 65, 73%) out of 89 smears prepared from suspected patients. Molecular analyses showed that the density of L. major was significantly higher (n= 48, 53.93%) than L. tropica (n= 4, 4.49%) (Mann-Whitney U test: p&lt; 0.05) and two samples (2.25%) remained ambiguous after several sequencing. L. major did not have diversity with two common haplotypes but L. tropica were found to exhibit high diversity with three novel haplotypes.  Conclusion L. major was considered the causative agent of leishmaniasis in the region, but the identification of a non-native L. tropica revealed the importance of further isolation of Leishmania parasites following molecular analyses and confirmation, and also revealed the importance of further isolation of Leishmania parasites from patients of the field areas who do not have easily access to health care centers for specialized treatment strategies.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000400278
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2018.07.005
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.22 n.4 2018
Fonte: | info:eu-repo/semantics/openAccess