Data/hora: 16/06/2024 15:48:18

Provedor de dados: | Brazilian Journal of Infectious Diseases
País: | Brazil
Título: | Genetic analysis of the first mcr-1 positive Escherichia coli isolate collected from an outpatient in Chile
Autores: | Gutiérrez,Camila; Zenis,Javier; Legarraga,Paulette; Cabrera-Pardo,Jaime R.; García,Patricia; Bello-Toledo,Helia; Opazo-Capurro,Andrés; González-Rocha,Gerardo.
Data: | 2019-06-01
Ano: | 2019
Palavras-chave: | Colistin-resistance; Mcr-1; Escherichia coli; Chile.
Resumo: | ABSTRACT Global dissemination of mcr-like genes represents a serious threat to public health since it jeopardizes the effectiveness of colistin, an antibiotic used as a last-resort treatment against highly antibiotic-resistant bacteria. In 2017, a mcr-1-positive isolate of Escherichia coli was found in Chile for the first time. Herein we report the genetic features of this strain (UCO-457) by whole-genome sequencing (WGS) and conjugation experiments. The UCO-457 strain belonged to ST4204 and carried a 285 kb IncI2-type plasmid containing the mcr-1 gene. Moreover, this plasmid was transferred by conjugation to an E. coli J53 strain at high frequency. The isolate harbored the cma, iroN, and iss virulence genes and did carry resistance genes to trimethoprim/sulfamethoxazole and fluoroquinolones. Other antibiotic resistance determinants such as β-lactamases-encoding genes were not detected, making the isolate highly susceptible to these antibiotics. Our results revealed that such susceptible isolates could be acting as platforms to disseminate plasmid-mediated colistin resistance. Based on this evidence, we consider that mcr-like prevalence deserves urgent attention and should be examined not only in highly resistant bacteria but also in susceptible isolates.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300203
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2019.05.008
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.23 n.3 2019
Fonte: | info:eu-repo/semantics/openAccess