Data/hora: 04/06/2024 00:14:55

Provedor de dados: 	55	
País: 	Brazil	
Título: 	High degree of concordance between ﬂow cytometry and geno2pheno methods for HIV-1 tropism determination in proviral DNA	
Autores: 	Torres,Alex José Leite; Brígido,Luis Fernando de Macedo; Abrahão,Marcos Herculano Nunes; Angelo,Ana Luiza Dias; Ferreira,Gilcivaldo de Jesus; Coelho,Luana Portes; Ferreira,João Leandro; Jorge,Célia Regina Mayoral Pedroso; Martins Netto,Eduardo; Brites,Carlos.	
Data: 	2015-04-01	
Ano: 	2015	
Palavras-chave: 	HIV-1; Tropism; Flow cytometry; Geno2pheno.	
Resumo: 	Use of CCR5 antagonists requires previous viral tropism determination. The available methods have high cost, are time-consuming, or require highly trained personnel, and sophisticated equipment. We compared a ﬂow cytometry-based tropism assay with geno2pheno method to determine HIV-1 tropism in AIDS patients, in Bahia, Brazil. We tested peripheral blood mononuclear cells of 102 AIDS patients under antiretroviral therapy by using a cytometry-based tropism assay and geno2pheno assay. Cellular membrane receptors were identiﬁed by using CXCR4, CCR5 and CD4 monoclonal antibodies, while detection of cytoplasmic mRNAs for gag and pol HIV regions was achieved by using a labeled probe. Genotypic identiﬁcation of X4 and R5 tropic viruses was attempted by geno2pheno algorithm. There was a high degree of concordance between cytometry-based tropism assay and geno2pheno algorithm in determination of HIV-1 tropism. Cytometry-based tropism assay demonstrated higher sensitivity and speciﬁcity in comparison to geno2pheno, which was used as a gold-standard. One sample could not be ampliﬁed by geno2pheno method, but was classiﬁed as duotropic by cytometry-based tropism assay. We did not ﬁnd any association between CD4+ count or plasma HIV-1 RNA viral load and tropism results. The overall performances of cytometry-based tropism assay and geno2pheno assay were almost identical in determination of HIV-1 tropism.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200163	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1016/j.bjid.2014.11.007	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.19 n.2 2015	
Fonte: 	info:eu-repo/semantics/openAccess