Data/hora: 10/06/2024 09:24:46

Provedor de dados: | Brazilian Journal of Infectious Diseases
País: | Brazil
Título: | Assessing and screening for T-cell epitopes from Mycobacterium tuberculosis RD2 proteins for the diagnosis of active tuberculosis
Autores: | Chen,Jiazhen; Ruan,Qiaoling; Shen,Yaojie; Wang,Sen; Shao,Lingyun; Zhang,Wenhong.
Data: | 2018-11-01
Ano: | 2018
Palavras-chave: | Mycobacterium tuberculosis; T-cell epitope; Diagnostic antigen.
Resumo: | ABSTRACT The Region of D eletion 2 (RD2) of Mycobacterium tuberculosis encodes reserved antigens that contribute to bacterial virulence. Among these antigens, Rv1983, Rv1986, Rv1987, and Rv1989c have been shown to be immunodominant in infected cattle; however, their diagnostic utility has not been evaluated in humans.In this study, we screened 87 overlapping synthetic peptides encoded by five RD2 proteins for diagnosing tuberculosis epitopes in 50 active tuberculosis (TB) cases, 31 non-tuberculosis patients and 36 healthy individuals. A pool of promising epitopes was then assessed for their diagnostic value in 233 suspected TB patients using a whole blood IFN-γ release assay.Only 10 peptides were recognized by more than 10% of active tuberculosis patients. The IFN-γ release responses to Rv1986-P9, P15, P16, Rv1988-P4, P11, and Rv1987-P11 were significantly higher in the active TB group than in the control groups (p &lt; 0.05). The whole blood IFN-γ release assay based on these epitopes yielded a sensitivity of 51% and a specificity of 85% in diagnosing active tuberculosis, and the corresponding results using the T-SPOT.TB assay were 76% and 75%, respectively.In conclusion, these results suggest that the six epitopes from the RD2 of M. tuberculosis have potential diagnostic value in TB.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600462
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2018.10.280
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.22 n.6 2018
Fonte: | info:eu-repo/semantics/openAccess