Data/hora: 17/06/2024 15:07:19

Provedor de dados: | 55
País: | Brazil
Título: | Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
Autores: | Chagas,Thiago Pavoni Gomes; Alves,Ronaldo Mendes; Vallim,Deyse Christina; Seki,Liliane Miyuki; Campos,Leila Carvalho; Asensi,Marise Dutra.
Data: | 2011-10-01
Ano: | 2011
Palavras-chave: | Klebsiella pneumoniae; Β-lactamases; Genotype.
Resumo: | OBJECTIVE: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. MATERIAL AND METHODS: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE. RESULTS AND DISCUSSION: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500002
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702011000500002
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.15 n.5 2011
Fonte: | info:eu-repo/semantics/openAccess