Data/hora: 09/06/2024 10:58:11

Provedor de dados: 	55	
País: 	Brazil	
Título: 	Association between the p53 arginine/arginine homozygous genotype at codon 72 and human papillomavirus E6/E7 mRNA expression	
Autores: 	Chuery,Ana Carolina Silva; Silva,Ismael Dale Cotrim Guerreiro da; Ribalta,Julisa Chamorro Lascasas; Speck,Neila Maria de Góis.	
Data: 	2017-06-01	
Ano: 	2017	
Palavras-chave: 	Human papillomavirus; Oncoproteins; Genetic polymorphisms; P53 gene; Arginine.	
Resumo: 	ABSTRACT  Objective: To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression.  Methods: We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS® EasyQ® HPV). The samples were divided into three groups: positive (n = 75) those with positive HPV E6/E7 mRNA expression and positive high-risk HPV Hybrid Capture (HR-HC) test; negative (n = 52) those with negative HPV E6/E7 mRNA expression and positive HR-HC; and control (n = 48) those with negative HPV E6/E7 mRNA expression and negative HR-HC. The p53 polymorphisms at codons 11, 72, and 248 were evaluated through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).  Results: The frequency of the arginine/arginine homozygous genotype at codon 72 was significantly higher in the positive (49.3%) than in the negative (32.7%) and control groups (20.8%, p = 0.002*). The frequency of the arginine allele was also significantly higher in the positive (67.3%) than in the negative (53.8%) and control groups (38.5%, p &lt; 0.001*). The arginine/arginine homozygous genotype was significantly associated with positive HPV E6/E7 mRNA expression (positive group) compared with negative and control groups (odds ratio: 2.633; 95% CI, 1.399-4.954, p = 0.003). The frequency of arginine/arginine homozygous genotype at codon 72 remained significantly more frequent in the positive group of women aged ≥30 years than in the other two groups.  Conclusion: The presence of the p53 arginine/arginine homozygous genotype at codon 72 was significantly associated with the positive HPV E6/E7 mRNA expression.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300248	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1016/j.bjid.2017.03.002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.21 n.3 2017	
Fonte: 	info:eu-repo/semantics/openAccess