Data/hora: 10/06/2024 11:59:47

Provedor de dados: 	Brazilian Journal of Infectious Diseases	
País: 	Brazil	
Título: 	Molecular diagnosis of cryptococcal meningitis in cerebrospinal ﬂuid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex	
Autores: 	Martins,Marilena dos Anjos; Brighente,Kate Bastos Santos; Matos,Terezinha Aparecida de; Vidal,Jose Ernesto; Hipólito,Daise Damaris Carnietto de; Pereira-Chioccola,Vera Lucia.	
Data: 	2015-02-01	
Ano: 	2015	
Palavras-chave: 	Cryptococcal meningitis; Acquired immunodeﬁciency syndrome; Polymerase chain reaction; Cerebrospinal ﬂuid.	
Resumo: 	Aim:This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples.Materials and methods:The sensitivity and speciﬁcity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattiispecies complex reference strains and 165 cere- brospinal ﬂuid samples from patients with neurological diseases divided into two groups: 96 patients with cryptococcal meningitis and AIDS; and 69 patients with other neurological opportunistic diseases (CRL/AIDS). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a speciﬁc product for C. neoformansand another for C. gattii).Results:CN4-CN5 primer set was positive in all Cryptococcusstandard strains and in 94.8% in DNA samples from cryptococcal meningitis and AIDS group. With the multiplex, no 448-bp product of C. gattiiwas observed in the clinical samples of either group. The 695 bp products of C. neoformanswere observed only in 64.6% of the cryptococcal meningitis and AIDS group. This primer set was negative for two standard strains. The speciﬁcity based on the negative samples from the CTL/AIDS group was 98.5% in both primer sets.Conclusions:These data suggest that the CN4/CN5 primer set was highly sensitive for the identiﬁcation of C. neoformans/C. gattiispecies complex in cerebrospinal ﬂuid samples from patients with clinical suspicion of cryptococcal meningitis.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000100062	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1016/j.bjid.2014.09.004	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.19 n.1 2015	
Fonte: 	info:eu-repo/semantics/openAccess