Data/hora: 09/06/2024 01:44:36

Provedor de dados: | 55
País: | Brazil
Título: | Molecular diagnosis of cryptococcal meningitis in cerebrospinal ﬂuid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex
Autores: | Martins,Marilena dos Anjos; Brighente,Kate Bastos Santos; Matos,Terezinha Aparecida de; Vidal,Jose Ernesto; Hipólito,Daise Damaris Carnietto de; Pereira-Chioccola,Vera Lucia.
Data: | 2015-02-01
Ano: | 2015
Palavras-chave: | Cryptococcal meningitis; Acquired immunodeﬁciency syndrome; Polymerase chain reaction; Cerebrospinal ﬂuid.
Resumo: | Aim:This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples.Materials and methods:The sensitivity and speciﬁcity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattiispecies complex reference strains and 165 cere- brospinal ﬂuid samples from patients with neurological diseases divided into two groups: 96 patients with cryptococcal meningitis and AIDS; and 69 patients with other neurological opportunistic diseases (CRL/AIDS). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a speciﬁc product for C. neoformansand another for C. gattii).Results:CN4-CN5 primer set was positive in all Cryptococcusstandard strains and in 94.8% in DNA samples from cryptococcal meningitis and AIDS group. With the multiplex, no 448-bp product of C. gattiiwas observed in the clinical samples of either group. The 695 bp products of C. neoformanswere observed only in 64.6% of the cryptococcal meningitis and AIDS group. This primer set was negative for two standard strains. The speciﬁcity based on the negative samples from the CTL/AIDS group was 98.5% in both primer sets.Conclusions:These data suggest that the CN4/CN5 primer set was highly sensitive for the identiﬁcation of C. neoformans/C. gattiispecies complex in cerebrospinal ﬂuid samples from patients with clinical suspicion of cryptococcal meningitis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000100062
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2014.09.004
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.19 n.1 2015
Fonte: | info:eu-repo/semantics/openAccess