Data/hora: 10/06/2024 11:05:42

Provedor de dados: 	55	
País: 	Brazil	
Título: 	Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum	
Autores: 	Khademvatan,Shahram; Gharavi,Mohammad Javad; Saki,Jasem.	
Data: 	2011-10-01	
Ano: 	2011	
Palavras-chave: 	Leishmania infantum; Apoptosis; Gene expression.	
Resumo: 	OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. MATERIALS AND METHODS: Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes expression of treated L. infantum were studied. RESULTS: Miltefosine led to dose-dependent death of L. infantumwith features compatible with apoptosis. Over expression of metacaspase and PARP was seen 6 hr after treatment. CONCLUSIONS: Our study showed that miltefosine exerts cytotoxic effect on L. infantum via an apoptotic-related mechanism.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500005	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1590/S1413-86702011000500005	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.15 n.5 2011	
Fonte: 	info:eu-repo/semantics/openAccess