Data/hora: 06/06/2024 02:45:22

Provedor de dados: 	Brazilian Journal of Infectious Diseases	
País: 	Brazil	
Título: 	Preservation of cytotoxic granule production in response to mycobacterial antigens by T-lymphocytes from vertically HIV-infected Brazilian youth on effective combined antiretroviral therapy	
Autores: 	Arrym,Mauro Pedromonico; Alves,Paulo César Martins; Castelhano,Mariana Virginello; Mazzola,Taís Nitsch; Lemos,Renata Muller Banzato Pinto de; Zaccariotto,Tânia Regina; Levy,Carlos Emilio; Guimarães,Fernando; Silva,Marcos Tadeu Nolasco da.	
Data: 	2019-06-01	
Ano: 	2019	
Palavras-chave: 	HIV/Aids; BCG; Youth; Tuberculosis; Citotoxicity; Immunologic.	
Resumo: 	ABSTRACT  Background: HIV infection harms adaptive cellular immunity mechanisms. Long-term virological control by combined antiretroviral therapy (cART) reduces the risk of mycobacterial infections. Thus, we aimed to study cellular responses to mycobacterial antigens in 20 HIV-infected adolescents with at least one year of virological control (HIV-RNA &lt;40 copies/mL) and 20 healthy adolescents.  Methods: We evaluated CD8 and γδ T-cell degranulation by measurement of CD107a membrane expression after stimulation with lysates from BCG (10 µg/mL) and H37RA Mycobacterium tuberculosis (Mtb, 10 µg/mL). Immune activation and antigen-presenting ability were also assessed by determination of HLA-DR, CD80, and CD86 markers.  Results: TCR γδ T-cell CD107a expression was similar between groups in response to mycobacterial antigens, and lower in the HIV-infected group in response to mitogen. Higher baseline HLA-DR expression and lower mycobacterial-stimulated expression was found within the HIV-infected group.  Conclusions: Similar degranulation in stimulated CD8+ and TCR γδ T-cells from HIV-infected adolescents, when compared to healthy controls suggests long-term immunological preservation with immune reconstitution under successful cART. However, differences in HLA-DR expression may represent ongoing inflammation and lower specific responses in HIV-infected youth. These features may be relevant in the context of the precocity and severity of vertically acquired HIV infection.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300151	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1016/j.bjid.2019.06.002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.23 n.3 2019	
Fonte: 	info:eu-repo/semantics/openAccess