Data/hora: 10/06/2024 18:16:01

Provedor de dados: 	Brazilian Journal of Infectious Diseases	
País: 	Brazil	
Título: 	Evaluation of the accuracy of various phenotypic tests to detect oxacillin resistance in coagulase-negative staphylococci	
Autores: 	Perez,Leandro Reus Rodrigues; d'Azevedo,Pedro Alves.	
Data: 	2008-06-01	
Ano: 	2008	
Palavras-chave: 	Oxacillin; Cefoxitin; CoNS.	
Resumo: 	In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30µg-cefoxitin and 1µg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. Of these, 138 (78.4%) harbored the mecA gene. Using 30µg-cefoxitin and 1µg-oxacillin disks we obtained 100 and 98.3% accuracy, respectively. In addition, when cefoxitin was used as marker in an agar dilution method, the higher accuracy (99.4%) was established with 8mg cefoxitin per mL breakpoint. Thus, despite of the agar dilution method using cefoxitin as a marker not being standard for this detection, our results suggested that it is an excellent alternative to detect the oxacillin resistance/mecA gene among CoNS isolates.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300009	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1590/S1413-86702008000300009	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.12 n.3 2008	
Fonte: 	info:eu-repo/semantics/openAccess