Data/hora: 08/06/2024 06:45:24

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Reduced hippocampal GABAergic function in Wistar audiogenic rats
Autores: | Drumond,L.E.; Kushmerick,C.; Guidine,P.A.M.; Doretto,M.C.; Moraes,M.F.D.; Massensini,A.R.
Data: | 2011-10-01
Ano: | 2011
Palavras-chave: | Epilepsy; Extracellular recording; GABA; Hippocampus; Wistar audiogenic rats; Picrotoxin.
Resumo: | Epilepsy is a neurological disorder associated with excitatory and inhibitory imbalance within the underlying neural network. This study evaluated inhibitory γ-amino-butyric acid (GABA)ergic modulation in the CA1 region of the hippocampus of male Wistar rats and Wistar audiogenic rats (aged 90 ± 3 days), a strain of inbred animals susceptible to audiogenic seizures. Field excitatory postsynaptic potentials and population spike complexes in response to Schaffer collateral fiber stimulation were recorded in hippocampal slices before and during application of picrotoxin (50 µM, 60 min), a GABA A antagonist, and the size of the population spike was quantified by measuring its amplitude and slope. In control audiogenic-resistant Wistar rats (N = 9), picrotoxin significantly increased both the amplitude of the population spike by 51 ± 19% and its maximum slope by 73 ± 21%. In contrast, in slices from Wistar audiogenic rats (N = 6), picrotoxin caused no statistically significant change in population spike amplitude (33 ± 46%) or slope (11 ± 29%). Data are reported as means ± SEM. This result indicates a functional reduction of GABAergic neurotransmission in hippocampal slices from Wistar audiogenic rats.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000011
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2011007500118
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.44 n.10 2011
Fonte: | info:eu-repo/semantics/openAccess