Data/hora: 16/06/2024 03:43:04

Provedor de dados: | 56
País: | Brazil
Título: | No evidence of association of MUC-1 genetic polymorphism with embryo implantation failure
Autores: | Dentillo,D.B.; Souza,F.R.P.; Meola,J.; Vieira,G.S.; Yazlle,M.E.H.D.; Goulart,L.R.; Martelli,L.
Data: | 2007-06-01
Ano: | 2007
Palavras-chave: | Mucin-1; Female infertility; Implantation failure; Variable number of tandem repeats; Genetic polymorphism.
Resumo: | Pregnancy loss can be caused by several factors involved in human reproduction. Although up to 50% of cases remain unexplained, it has been postulated that the major cause of failed pregnancy is an error of embryo implantation. Transmembrane mucin-1 (MUC-1) is a glycoprotein expressed on the endometrial cell surface which acts as a barrier to implantation. The gene that codes for this molecule is composed of a polymorphic tandem repeat of 60 nucleotides. Our objective was to determine if MUC-1 genetic polymorphism is associated with implantation failure in patients with a history of recurrent abortion. The study was conducted on 10 women aged 25 to 35 years with no history of successful pregnancy and with a diagnosis of infertility. The control group consisted of 32 patients aged 25 to 35 years who had delivered at least two full-term live children and who had no history of abortions or fetal losses. MUC-1 amplicons were obtained by PCR and observed on agarose and polyacrylamide gel after electrophoresis. Statistical analysis showed no significant difference in the number of MUC-1 variable number of tandem repeats between these groups (P &gt; 0.05). Our results suggest that there is no effect of the polymorphic MUC-1 sequence on the implantation failure. However, the data do not exclude MUC-1 relevance during embryo implantation. The process is related to several associated factors such as the mechanisms of gene expression in the uterus, specific MUC-1 post-translational modifications and appropriate interactions with other molecules during embryo implantation.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600007
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2007000600007
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.40 n.6 2007
Fonte: | info:eu-repo/semantics/openAccess