Data/hora: 10/06/2024 06:39:11

Provedor de dados: 	Brazilian Journal of Medical and Biological Research	
País: 	Brazil	
Título: 	In vitro proliferation and differentiation of hepatic oval cells and their potential capacity for intrahepatic transplantation	
Autores: 	Li,Z.; Chen,J.; Li,L.; Ran,J.H.; Liu,J.; Gao,T.X.; Guo,B.Y.; Li,X.H.; Liu,Z.H.; Liu,G.J.; Gao,Y.C.; Zhang,X.L.	
Data: 	2013-08-01	
Ano: 	2013	
Palavras-chave: 	Hepatic oval cells; Proliferation; Differentiation; Liver transplantation.	
Resumo: 	Hepatic oval cells (HOCs) are recognized as facultative liver progenitor cells that play a role in liver regeneration after acute liver injury. Here, we investigated the in vitro proliferation and differentiation characteristics of HOCs in order to explore their potential capacity for intrahepatic transplantation. Clusters or scattered HOCs were detected in the portal area and interlobular bile duct in the liver of rats subjected to the modified 2-acetylaminofluorene and partial hepatectomy method. Isolated HOCs were positive for c-kit and CD90 staining (99.8% and 88.8%, respectively), and negative for CD34 staining (3.6%) as shown by immunostaining and flow cytometric analysis. In addition, HOCs could be differentiated into hepatocytes and bile duct epithelial cells after leukemia inhibitory factor deprivation. A two-cuff technique was used for orthotopic liver transplantation, and HOCs were subsequently transplanted into recipients. Biochemical indicators of liver function were assessed 4 weeks after transplantation. HOC transplantation significantly prolonged the median survival time and improved the liver function of rats receiving HOCs compared to controls (P=0.003, Studentt-test). Administration of HOCs to rats also receiving liver transplantation significantly reduced acute allograft rejection compared to control liver transplant rats 3 weeks following transplantation (rejection activity index score: control=6.3±0.9; HOC=3.5±1.5; P=0.005). These results indicate that HOCs may be useful in therapeutic liver regeneration after orthotopic liver transplantation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800681	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431X20132620	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.46 n.8 2013	
Fonte: 	info:eu-repo/semantics/openAccess