Data/hora: 10/06/2024 01:35:46

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Mini-Mu insertions in the tetracycline resistance determinant from Proteus mirabilis	
Data: 	1997-03-01	
Ano: 	1997	
Palavras-chave: 	Tetracycline; Mutation; LacZ-fusion; Proteus mirabilis; Bacteriophage Mu.	
Resumo: 	The inducible tetracycline resistance determinant isolated from Proteus mirabilis cloned into the plasmid pACYC177 was mutagenized by insertion of a mini-Mu-lac phage in order to define the regions in the cloned sequences encoding the structural and regulatory proteins. Three different types of mutants were obtained: one lost the resistance phenotype and became Lac+; another expressed the resistance at lower levels and constitutively; the third was still dependent on induction but showed a lower minimal inhibitory concentration. The mutant phenotypes and the locations of the insertions indicate that the determinant is composed of a repressor gene and a structural gene which are not transcribed divergently as are other known tetracycline determinants isolated from Gram-negative bacteria	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300009	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X1997000300009	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.30 n.3 1997	
Fonte: 	info:eu-repo/semantics/openAccess