Data/hora: 03/06/2024 16:25:52

Provedor de dados: | 56
País: | Brazil
Título: | Effects of microcystin-LR in isolated perfused rat kidney
Autores: | Nobre,A.C.L.; Jorge,M.C.M.; Menezes,D.B.; Fonteles,M.C.; Monteiro,H.S.A.
Data: | 1999-08-01
Ano: | 1999
Palavras-chave: | Microcystin-LR; Kidney; Perfused kidney.
Resumo: | Microcystin is a hepatotoxic peptide which inhibits protein phosphatase types 1 and 2A. The objective of the present study was to evaluate the physiopathologic effects of microcystin-LR in isolated perfused rat kidney. Adult Wistar rats (N = 5) of both sexes (240-280 g) were utilized. Microcystin-LR (1 µg/ml) was perfused over a period of 120 min, during which samples of urine and perfusate were collected at 10-min intervals to determine the levels of inulin, sodium, potassium and osmolality. We observed a significant increase in urinary flow with a peak effect at 90 min (control (C) = 0.20 ± 0.01 and treated (T) = 0.32 ± 0.01 ml g-1 min-1, P&amp;lt;0.05). At 90 min there was a significant increase in perfusate pressure (C = 129.7 ± 4.81 and T = 175.0 ± 1.15 mmHg) and glomerular filtration rate (C = 0.66 ± 0.07 and T = 1.10 ± 0.04 ml g-1 min-1) and there was a significant reduction in fractional sodium tubular transport at 120 min (C = 78.6 ± 0.98 and T = 73.9 ± 0.95%). Histopathologic analysis of the perfused kidneys showed protein material in the urinary space, suggestive of renal toxicity. These data demonstrate renal vascular, glomerular and urinary effects of microcystin-LR, indicating that microcystin acts directly on the kidney by probable inhibition of protein phosphatases.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999000800008
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.8 1999
Fonte: | info:eu-repo/semantics/openAccess