Data/hora: 10/06/2024 17:17:51

Provedor de dados: 	Brazilian Journal of Medical and Biological Research	
País: 	Brazil	
Título: 	Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples	
Autores: 	Santos,M.F.M.; Oliveira,F.C.A.C.; Kishimoto,R.K.; Borri,D.; Santos,F.P.S.; Campregher,P.V.; Silveira,P.A.A.; Hamerschlak,N.; Mangueira,C.L.P.; Duarte,F.B.; Crepaldi,A.H.; Salvino,M.A.; Velloso,E.D.R.P.	
Data: 	2019-01-01	
Ano: 	2019	
Palavras-chave: 	Cytogenetics; Karyotype; Leukemia; Myelodysplasia; Myeloproliferative disease.	
Resumo: 	Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/1414-431x20188194	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.52 n.2 2019	
Fonte: 	info:eu-repo/semantics/openAccess