Data/hora: 02/06/2024 03:59:31

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
Autores: | Santos,M.F.M.; Oliveira,F.C.A.C.; Kishimoto,R.K.; Borri,D.; Santos,F.P.S.; Campregher,P.V.; Silveira,P.A.A.; Hamerschlak,N.; Mangueira,C.L.P.; Duarte,F.B.; Crepaldi,A.H.; Salvino,M.A.; Velloso,E.D.R.P.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Cytogenetics; Karyotype; Leukemia; Myelodysplasia; Myeloproliferative disease.
Resumo: | Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200608
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20188194
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.52 n.2 2019
Fonte: | info:eu-repo/semantics/openAccess