Data/hora: 10/06/2024 03:43:47

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | The chronic blockade of angiotensin I-converting enzyme eliminates the sex differences of serum cytokine levels of spontaneously hypertensive rats
Autores: | Dalpiaz,P.L.M.; Lamas,A.Z.; Caliman,I.F.; Medeiros,A.R.S.; Abreu,G.R.; Moysés,M.R.; Andrade,T.U.; Alves,M.F.; Carmona,A.K.; Bissoli,N.S.
Data: | 2013-02-01
Ano: | 2013
Palavras-chave: | SHR Enalapril Sexual dimorphism Angiotensin I-converting enzyme Cytokines IL-10; IL-6 and TNF-α.
Resumo: | Sex hormones modulate the action of both cytokines and the renin-angiotensin system. However, the effects of angiotensin I-converting enzyme (ACE) on the proinflammatory and anti-inflammatory cytokine levels in male and female spontaneously hypertensive rats (SHR) are unclear. We determined the relationship between ACE activity, cytokine levels and sex differences in SHR. Female (F) and male (M) SHR were divided into 4 experimental groups each (n = 7): sham + vehicle (SV), sham + enalapril (10 mg/kg body weight by gavage), castrated + vehicle, and castrated + enalapril. Treatment began 21 days after castration and continued for 30 days. Serum cytokine levels (ELISA) and ACE activity (fluorimetry) were measured. Male rats exhibited a higher serum ACE activity than female rats. Castration reduced serum ACE in males but did not affect it in females. Enalapril reduced serum ACE in all groups. IL-10 (FSV = 16.4 ± 1.1 pg/mL; MSV = 12.8 ± 1.2 pg/mL), TNF-α (FSV = 16.6 ± 1.2 pg/mL; MSV = 12.8 ± 1 pg/mL) and IL-6 (FSV = 10.3 ± 0.2 pg/mL; MSV = 7.2 ± 0.2 pg/mL) levels were higher in females than in males. Ovariectomy reduced all cytokine levels and orchiectomy reduced IL-6 but increased IL-10 concentrations in males. Castration eliminated the differences in all inflammatory cytokine levels (IL-6 and TNF-α) between males and females. Enalapril increased IL-10 in all groups and reduced IL-6 in SV rats. In conclusion, serum ACE inhibition by enalapril eliminated the sexual dimorphisms of cytokine levels in SV animals, which suggests that enalapril exerts systemic anti-inflammatory and anti-hypertensive effects.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000200171
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20122472
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.46 n.2 2013
Fonte: | info:eu-repo/semantics/openAccess