Data/hora: 11/06/2024 05:43:59

Provedor de dados: | 56
País: | Brazil
Título: | BMP-2 and titanium particles synergistically activate osteoclast formation
Autores: | Sun,S.X.; Guo,H.H.; Zhang,J.; Yu,B.; Sun,K.N.; Jin,Q.H.
Data: | 2014-06-01
Ano: | 2014
Palavras-chave: | Bone morphogenetic protein; Titanium; Osteoclasts; SMAD; RANKL.
Resumo: | A previous study showed that BMP-2 (bone morphogenetic protein-2) and wear debris can separately support osteoclast formation induced by the receptor activator of NF-κB ligand (RANKL). However, the effect of BMP-2 on wear debris-induced osteoclast formation is unclear. In this study, we show that neither titanium particles nor BMP-2 can induce osteoclast formation in RAW 264.7 mouse leukemic monocyte macrophage cells but that BMP-2 synergizes with titanium particles to enhance osteoclast formation in the presence of RANKL, and that at a low concentration, BMP-2 has an optimal effect to stimulate the size and number of multinuclear osteoclasts, expression of osteoclast genes, and resorption area. Our data also clarify that the effects caused by the increase in BMP-2 on phosphorylated SMAD levels such as c-Fos expression increased throughout the early stages of osteoclastogenesis. BMP-2 and titanium particles stimulate the expression of p-JNK, p-P38, p-IkB, and P50 compared with the titanium group. These data suggested that BMP-2 may be a crucial factor in titanium particle-mediated osteoclast formation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600461
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20132966
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.47 n.6 2014
Fonte: | info:eu-repo/semantics/openAccess