Data/hora: 08/06/2024 19:00:24

Provedor de dados: 	56	
País: 	Brazil	
Título: 	IL-6 upregulation contributes to the reduction of miR-26a expression in hepatocellular carcinoma cells	
Autores: 	Zhang,Yafei; Zhang,Bicheng; Zhang,Anran; Li,Xiaohua; Liu,Jian; Zhao,Jie; Zhao,Yong; Gao,Jianfei; Fang,Dianchun; Rao,Zhiguo.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	MiR-26a; IL-6; C-Myc; Hepatocellular carcinoma; Carcinoma prognosis.	
Resumo: 	A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be a predictor of survival up to 5 years for hepatocellular carcinoma patients (log-rank test, P &lt; 0.05). We observed that the serum IL-6 concentration was inversely correlated with miR-26a expression in cancerous tissues (Pearson correlation test, r = -0.651, P &lt; 0.01). Furthermore, by in vitro experiments with HepG2 cells, we showed that IL-6 stimulation can lead to miR-26a suppression via c-Myc activation, whereas in normal hepatocyte LO2 cells incubation with IL-6 had no significant effect on miR-26a expression. Taken together, these results indicate that miR-26a reduction in hepatocellular carcinoma might be due to IL-6 upregulation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X2012007500155	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.46 n.1 2013	
Fonte: 	info:eu-repo/semantics/openAccess