Data/hora: 16/06/2024 03:20:49

Provedor de dados: | 56
País: | Brazil
Título: | Upregulation of microRNA 142-3p in the peripheral blood and urinary cells of kidney transplant recipients with post-transplant graft dysfunction
Autores: | Domenico,T.D.; Joelsons,G.; Montenegro,R.M.; Manfro,R.C.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | MicroRNA; Graft dysfunction; Renal transplantation; Gene expression; Biomarker.
Resumo: | We analyzed microRNA (miR)-142-3p expression in leucocytes of the peripheral blood and urinary sediment cell samples obtained from kidney transplant recipients who developed graft dysfunction. Forty-one kidney transplant recipients with kidney graft dysfunction and 8 stable patients were included in the study. The groups were divided according to histological analysis into acute rejection group (n=23), acute tubular necrosis group (n=18) and stable patients group used as a control for gene expression (n=8). Percutaneous biopsies were performed and peripheral blood samples and urine samples were obtained. miR-142-3p was analyzed by real-time polymerase chain reaction. The group of patients with acute tubular necrosis presented significantly higher expressions in peripheral blood (P&lt;0.05) and urine (P&lt;0.001) compared to the stable patients group. Also, in the peripheral blood, miR-142-3p expression was significantly higher in the acute tubular necrosis group compared to the acute rejection group (P&lt;0.05). Urine samples of the acute rejection group presented higher expression compared to the stable patients group (P&lt;0.001) but the difference between acute tubular necrosis and acute rejection groups was not significant in the urinary analyzes (P=0.079). miR-142-3p expression has a distinct pattern of expression in the setting of post-operative acute tubular necrosis after kidney transplantation and may potentially be used as a non-invasive biomarker for renal graft dysfunction.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400605
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20175533
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.50 n.4 2017
Fonte: | info:eu-repo/semantics/openAccess