Data/hora: 08/06/2024 10:48:59

Provedor de dados: | 56
País: | Brazil
Título: | Immune response to respiratory syncytial virus in young Brazilian children
Autores: | Queiróz,D.A.O.; Durigon,E.L.; Botosso,V.F.; Ejzemberg,B.; Vieira,S.E.; Mineo,J.R.; Yamashita,C.; Hein,N.; Lopes,C.L.; Cacharo,A.L.; Stewien,K.E.
Data: | 2002-10-01
Ano: | 2002
Palavras-chave: | Respiratory syncytial virus; Peripheral blood mononuclear cells; Young children; Surface antigens; Cytokines.
Resumo: | We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients &lt;=12 months of age (39 males and 26 females, 28 cases &lt;3 months and 37 cases  &gt; or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children &lt;3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children &gt;3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2002001000011
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.35 n.10 2002
Fonte: | info:eu-repo/semantics/openAccess