Data/hora: 11/06/2024 02:29:22

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Modulation of murine blastocyst hatching in vitro by glutamine and tryptophan
Autores: | Castro,W.; Bueno Sánchez,J.C.; Piedrahita-Ochoa,C.; Cadavid J.,A.P.
Data: | 2011-08-01
Ano: | 2011
Palavras-chave: | Embryo development; Murine blastocyst; Hatching; Glutamine; Tryptophan.
Resumo: | Enrichment of culture media with amino acids improves embryo development. However, little is known about the specific action of each amino acid during embryogenesis. The present study was undertaken to examine the effect of L-glutamine (Gln) and tryptophan (Trp) on mouse embryo hatching, expansion and viability in vitro. Blastocysts were collected from 6- to 8-week-old female BALB/c mice (N = 30) and cultured in M2 medium containing either 0.125, 0.25 or 0.5 mM Trp, 1 mM Gln, or M2 alone. Gln significantly increased (100%; P &lt; 0.05) blastocyst hatching at 24 h compared to M2 alone or Trp; moreover, Trp inhibited blastocyst hatching when compared to M2 alone (P &lt; 0.05) at 72 h. In contrast, the percentage of embryos reaching the state of expanded blastocyst at 48 h was significantly higher in medium with 1 mM Gln (66.6%; P &lt; 0.05) or with 0.125 mM Trp (61.1%; P &lt; 0.05). Unexpectedly, Trp increased the percentage of degenerated blastocysts after 48 h (67.7%; P &lt; 0.05), while Gln preserved blastocyst viability. These results suggest that Gln may enhance blastocyst hatching, expansion and viability in vitro.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800003
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2011007500080
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.44 n.8 2011
Fonte: | info:eu-repo/semantics/openAccess