Data/hora: 01/06/2024 18:46:10

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Effects of a neuronal nitric oxide synthase inhibitor on lipopolysaccharide-induced fever
Autores: | Perotti,C.A.A.; Nogueira,M.S.; Antunes-Rodrigues,J.; Cárnio,E.C.
Data: | 1999-11-01
Ano: | 1999
Palavras-chave: | Nitric oxide; 7-nitroindazole; Body temperature; LPS; L-NAME.
Resumo: | It has been demonstrated that nitric oxide (NO) has a thermoregulatory action, but very little is known about the mechanisms involved. In the present study we determined the effect of neuronal nitric oxide synthase (nNOS) inhibition on thermoregulation. We used 7-nitroindazole (7-NI, 1, 10 and 30 mg/kg body weight), a selective nNOS inhibitor, injected intraperitoneally into normothermic Wistar rats (200-250 g) and rats with fever induced by lipopolysaccharide (LPS) (100 µg/kg body weight) administration. It has been demonstrated that the effects of 30 mg/kg of 7-NI given intraperitoneally may inhibit 60% of nNOS activity in rats. In all experiments the colonic temperature of awake unrestrained rats was measured over a period of 5 h at 15-min intervals after intraperitoneal injection of 7-NI. We observed that the injection of 30 mg/kg of 7-NI induced a 1.5oC drop in body temperature, which was statistically significant 1 h after injection (P&amp;lt;0.02). The coinjection of LPS and 7-NI was followed by a significant (P&amp;lt;0.02) hypothermia about 0.5oC below baseline. These findings show that an nNOS isoform is required for thermoregulation and participates in the production of fever in rats.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999001100008
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.11 1999
Fonte: | info:eu-repo/semantics/openAccess