Data/hora: 10/06/2024 08:45:16

Provedor de dados: | 56
País: | Brazil
Título: | Identification of Mycobacterium bovis antigens by analysis of bovine T-cell responses after infection with a virulent strain
Autores: | Alito,A.; McNair,J.; Girvin,R.M.; Zumarraga,M.; Bigi,F.; Pollock,J.M.; Cataldi,A.
Data: | 2003-11-01
Ano: | 2003
Palavras-chave: | Bovine tuberculosis; Antigens; Mycobacterium bovis; Cellular immunity.
Resumo: | Purification and characterization of individual antigenic proteins are essential for the understanding of the pathogenic mechanisms of mycobacteria and the immune response against them. In the present study, we used anion-exchange chromatography to fractionate cell extracts and culture supernatant proteins from Mycobacterium bovis to identify T-cell-stimulating antigens. These fractions were incubated with peripheral blood mononuclear cells (PBMC) from M. bovis-infected cattle in lymphoproliferation assays. This procedure does not denature proteins and permits the testing of mixtures of potential antigens that could be later identified. We characterized protein fractions with high stimulation indices from both culture supernatants and cell extracts. Proteins were identified by two-dimensional gel electrophoresis followed by N-terminal sequencing or MALDI-TOF. Culture supernatant fractions containing low molecular weight proteins such as ESAT6 and CFP10 and other proteins (85B, MPB70), and the novel antigens TPX and TRB-B were associated with a high stimulation index. These results reinforce the concept that some low molecular weight proteins such as ESAT6 and CFP10 play an important role in immune responses. Also, Rv3747 and L7/L12 were identified in high stimulation index cell extract fractions. These data show that protein fractions with high lymphoproliferative activity for bovine PBMC can be characterized and antigens which have been already described and new protein antigens can also be identified in these fractions.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100011
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2003001100011
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.36 n.11 2003
Fonte: | info:eu-repo/semantics/openAccess