Data/hora: 02/06/2024 13:49:28

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells
Autores: | Chen,W.L.; Luo,D.F.; Gao,C.; Ding,Y.; Wang,S.Y.
Data: | 2015-07-01
Ano: | 2015
Palavras-chave: | FAMLF; Gene expression; Leukemia; Real-time polymerase chain reaction; Alternative splicing.
Resumo: | The familial acute myeloid leukemia related factor gene (FAMLF) was previously identified from a familial AML subtractive cDNA library and shown to undergo alternative splicing. This study used real-time quantitative PCR to investigate the expression of the FAMLF alternative-splicing transcript consensus sequence (FAMLF-CS) in peripheral blood mononuclear cells (PBMCs) from 119 patients with de novo acute leukemia (AL) and 104 healthy controls, as well as in CD34+ cells from 12 AL patients and 10 healthy donors. A 429-bp fragment from a novel splicing variant of FAMLF was obtained, and a 363-bp consensus sequence was targeted to quantify total FAMLF expression. Kruskal-Wallis, Nemenyi, Spearman's correlation, and Mann-Whitney U-tests were used to analyze the data. FAMLF-CS expression in PBMCs from AL patients and CD34+ cells from AL patients and controls was significantly higher than in control PBMCs (P&lt;0.0001). Moreover, FAMLF-CS expression in PBMCs from the AML group was positively correlated with red blood cell count (rs =0.317, P=0.006), hemoglobin levels (rs =0.210, P=0.049), and percentage of peripheral blood blasts (rs =0.256, P=0.027), but inversely correlated with hemoglobin levels in the control group (rs =–0.391, P&lt;0.0001). AML patients with high CD34+ expression showed significantly higher FAMLF-CS expression than those with low CD34+ expression (P=0.041). Our results showed that FAMLF is highly expressed in both normal and malignant immature hematopoietic cells, but that expression is lower in normal mature PBMCs.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700603
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20154430
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.48 n.7 2015
Fonte: | info:eu-repo/semantics/openAccess