Data/hora: 17/06/2024 02:15:59

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Immune response induced in mice oral immunization with cowpea severe mosaic virus
Autores: | Florindo,M.I.; Aragão,M.E.F. de; Silva,A.C.M. da; Otoch,M.L.; Melo,D. Fernandes de; Lima,J.A.A.; Lima,M.G. Silva.
Data: | 2002-07-01
Ano: | 2002
Palavras-chave: | Oral immunization; Plant virus; Plant virus immunogenicity; Cowpea severe mosaic virus.
Resumo: | There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera diluted at least 1:400 at days 7, 14, 21, 28, 35 and 42 after the 10th dose. IgG and IgA against CPSMV were produced systemically, but IgE was not detected. No synthesis of specific antibodies against the proteins of leaf extracts from V. unguiculata, infected or not with CPSMV, was detected. The use of CPSMV, a plant-infecting virus that apparently does not induce a pathogenic response in animals, induced a humoral and persistent (at least 6 months) immune response through the administration of low antigen doses by gavage. These results raise the possibility of using CPSMV either as a vector for the production of vaccines against animal pathogens or in quick and easy methods to produce specific antisera for viral diagnosis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700011
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2002000700011
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.35 n.7 2002
Fonte: | info:eu-repo/semantics/openAccess