Data/hora: 17/06/2024 15:30:59

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain	
Autores: 	Miranda,E.A.; de-Murcia,G.; Ménissier-de-Murcia,J.	
Data: 	1997-08-01	
Ano: 	1997	
Palavras-chave: 	PARP; Insect cells; Baculovirus; Overproduction.	
Resumo: 	A simple and inexpensive shaker/Erlenmeyer flask system for large-scale cultivation of insect cells is described and compared to a commercial spinner system. On the basis of maximum cell density, average population doubling time and overproduction of recombinant protein, a better result was obtained with a simpler and less expensive bioreactor consisting of Erlenmeyer flasks and an ordinary shaker waterbath. Routinely, about 90 mg of pure poly(ADP-ribose) polymerase catalytic domain was obtained for a total of 3 x 109 infected cells in three liters of culture	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800002	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X1997000800002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.30 n.8 1997	
Fonte: 	info:eu-repo/semantics/openAccess