Data/hora: 09/06/2024 17:56:07

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Modulation of the expression of the transcription factor Max in rat retinal ganglion cells by a recombinant adeno-associated viral vector
Autores: | Petrs-Silva,H.; Chiodo,V.; Chiarini,L.B.; Hauswirth,W.W.; Linden,R.
Data: | 2005-03-01
Ano: | 2005
Palavras-chave: | Retina; Max expression; Transcription factor; Viral vector; Gene therapy; Neuroprotection.
Resumo: | Exclusion of the transcription factor Max from the nucleus of retinal ganglion cells is an early, caspase-independent event of programmed cell death following damage to the optic axons. To test whether the loss of nuclear Max leads to a reduction in neuroprotection, we developed a procedure to overexpress Max protein in rat retinal tissue in vivo. A recombinant adeno-associated viral vector (rAAV) containing the max gene was constructed, and its efficiency was confirmed by transduction of HEK-293 cells. Retinal ganglion cells were accessed in vivo through intravitreal injections of the vector in rats. Overexpression of Max in ganglion cells was detected by immunohistochemistry at 2 weeks following rAAV injection. In retinal explants, the preparation of which causes damage to the optic axons, Max immunoreactivity was increased after 30 h in vitro, and correlated with the preservation of a healthy morphology in ganglion cells. The data show that the rAAV vector efficiently expresses Max in mammalian retinal ganglion cells, and support the hypothesis that the Max protein plays a protective role for retinal neurons.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2005000300008
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.38 n.3 2005
Fonte: | info:eu-repo/semantics/openAccess