Data/hora: 16/06/2024 00:08:17

Provedor de dados: | 56
País: | Brazil
Título: | Interferon-alpha receptor 1 mRNA expression in peripheral blood mononuclear cells is associated with response to interferon-alpha therapy of patients with chronic hepatitis C
Autores: | Massirer,K.B.; Hirata,M.H.; Silva,A.E.B.; Ferraz,M.L.G.; Nguyen,N.Y.; Hirata,R.D.C.
Data: | 2004-05-01
Ano: | 2004
Palavras-chave: | IFNAR1-mRNA expression; Interferon-alpha therapy; Chronic hepatitis C; Interferon-alpha receptor; Peripheral blood mononuclear cells; Reverse transcription- polymerase chain reaction.
Resumo: | Interferon (IFN)-alpha receptor mRNA expression in liver of patients with chronic hepatitis C has been shown to be a response to IFN-alpha therapy. The objective of the present study was to determine whether the expression of mRNA for subunit 1 of the IFN-alpha receptor (IFNAR1) in peripheral blood mononuclear cells (PBMC) is associated with the response to IFN-alpha in patients with chronic hepatitis C. Thirty patients with positive anti-HCV and HCV-RNA, and abnormal levels of alanine aminotransferase in serum were selected and treated with IFN-alpha2b for one year. Those with HBV or HIV infection, or using alcohol were not included. Thirteen discontinued the treatment and were not evaluated. The IFN-alpha response was monitored on the basis of alanine aminotransferase level and positivity for HCV-RNA in serum. IFNAR1-mRNA expression in PBMC was measured by reverse transcription-polymerase chain reaction before and during the first three months of therapy. The results are reported as IFNAR1-mRNA/ß-actin-mRNA ratio (mean ± SD). Before treatment, responder patients had significantly higher IFNAR1-mRNA expression in PBMC (0.67 ± 0.15; N = 5; P &lt; 0.05) compared to non-responders (0.35 ± 0.17; N = 12) and controls (0.30 ± 0.16; N = 9). Moreover, IFNAR1-mRNA levels were significantly reduced after 3 months of treatment in responders, whereas there were no differences in IFNAR1 expression in non-responders during IFN-alpha therapy. Basal IFNAR1-mRNA expression was not correlated with the serum level of alanine and aspartate aminotransferases or the presence of cirrhosis. The present results suggest that IFNAR1-mRNA expression in PBMC is associated with IFN-alpha response to hepatitis C and may be useful for monitoring therapy in patients with chronic hepatitis C.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500003
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2004000500003
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.37 n.5 2004
Fonte: | info:eu-repo/semantics/openAccess