Data/hora: 10/06/2024 12:13:07

Provedor de dados: | 56
País: | Brazil
Título: | Mechanism of hif-1α mediated hypoxia-induced permeability changes in bladder endothelial cells
Autores: | Liu,C.; Shui,C.L.; Wang,Q.; Luo,H.; Gu,C.G.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Bladder cancer; Hypoxia inducible factor-1α; VEGF; Cell permeability; YC-1.
Resumo: | This study aimed to investigate the mechanism of hypoxia-inducible factor-1 alpha (HIF-1α) mediated hypoxia-induced permeability changes in bladder endothelial cells. Models of in vitro hypoxic cell culture of bladder cancer, bladder cancer cells with low HIF-1α expression and HIF-1α RNA interference (RNAi) expression vector were established. Western blot and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of HIF-1α and vascular endothelial growth factor (VEGF) in each group. Bladder cell permeability was determined. Results showed that protein and mRNA expression of HIF-1α and VEGF at 3 and 12 h of hypoxia were significantly higher than normal control (P&lt;0.05), and peaked at 12 h. HIF-1α and VEGF expression in the hypoxic group and hypoxic+3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1) group were significantly higher than normal control (P&lt;0.05), while expression in the hypoxic+YC-1 group was significantly lower than the hypoxic group (P&lt;0.05). Bladder cell permeability in the hypoxic and hypoxic+YC-1 group were significantly increased compared to normal control (P&lt;0.05), while in the hypoxic+YC-1 group was significantly decreased compared to the hypoxic group (P&lt;0.05). Most of the cells in the stably transfected HIF-1α RNAi expression vector pcDNA6.2-GW/EmGFP-miR-siHIF-1α expressed green fluorescence protein (GFP) under fluorescence microscope. pcDNA6.2-GW/EmGFP-miR-siHIF-1α could significantly inhibit HIF-1α gene expression (P&lt;0.05). HIF-1α and VEGF expression in the hypoxic group and siHIF-1α hypoxic group were significantly higher than normal group (P&lt;0.05), while expression in the siHIF-1α hypoxic group was significantly lower than the hypoxic group (P&lt;0.05). Findings suggest that HIF-1α is an important factor in the increase of bladder cancer cell permeability.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200616
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20176768
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.51 n.2 2018
Fonte: | info:eu-repo/semantics/openAccess