Data/hora: 16/06/2024 06:45:54

Provedor de dados: | 56
País: | Brazil
Título: | Fibrinolytic action on fresh human clots of whole body extracts and two semipurified fractions from Lonomia achelous caterpillar
Autores: | Coll-Sangrona,E.; Arocha-Piñango,C.L.
Data: | 1998-06-01
Ano: | 1998
Palavras-chave: | Lonomia achelous caterpillars; Fibrinolysis; Clot lysis; Plasmin.
Resumo: | The severe bleeding diathesis produced by intoxication with the venom of Lonomia achelous caterpillars is characterized by prolonged bleeding from superficial skin wounds as well as massive hemorrhage into body cavities. The aim of the present study was to evaluate the effect of the crude venom and its fibrinolytic fractions on in vitro lysis of whole blood clots. Venom fractions with fibrinolytic activity were obtained by gel filtration chromatography on Sephadex G75 using imidazole buffer, pH 7.4, at a flow rate of 24 ml/h. Four peaks with fibrinolytic activity were obtained by this method. The highest activity was found in the first two peaks (both peaks were used for the experiments). The results show that the caterpillar venom degraded the preformed clots at a slower rate than plasmin. In addition, plasma protease inhibitors of the fibrinolytic system (&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2-antiplasmin, &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;2-macroglobulin, PAI, etc.) only weakly inhibited the lytic effect of the caterpillar venom. These characteristics, as well as the pattern of fibrinogen degradation products, the delay period on fibrin plate lysis and amidolytic activity on chromogenic substrate, reported previously, indicate that the caterpillar enzymes are different from plasmin and trypsin.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600009
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1998000600009
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.31 n.6 1998
Fonte: | info:eu-repo/semantics/openAccess