Data/hora: 09/06/2024 07:40:42

Provedor de dados: | 56
País: | Brazil
Título: | Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts
Autores: | Zhang,L.; Ding,Y.; Rao,G.Z.; Miao,D.
Data: | 2016-01-01
Ano: | 2016
Palavras-chave: | Human periodontal ligament fibroblasts; Interleukin-10; Glucose; Osteoprotegerin; Receptor activator of nuclear factor-κB ligand.
Resumo: | The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P&lt;0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000400605
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20154324
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.49 n.4 2016
Fonte: | info:eu-repo/semantics/openAccess