Data/hora: 18/06/2024 06:17:13

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Expression of a hantavirus N protein and its efficacy as antigen in immune assays
Autores: | Figueiredo,L.T.M.; Moreli,M.L.; Borges,A.A.; Figueiredo,G.G.; Souza,R.L.M.; Aquino,V.H.
Data: | 2008-07-01
Ano: | 2008
Palavras-chave: | Diagnosis; Hantavirus infection; Araraquara hantavirus; N protein.
Resumo: | Hantavirus cardiopulmonary syndrome (HCPS) has been recognized as an important public heath problem. Five hantaviruses associated with HCPS are currently known in Brazil: Juquitiba, Araraquara, Laguna Negra-like, Castelo dos Sonhos, and Anajatuba viruses. The laboratory diagnosis of HCPS is routinely carried out by the detection of anti-hantavirus IgM and/or IgG antibodies. The present study describes the expression of the N protein of a hantavirus detected in the blood sample of an HCPS patient. The entire S segment of the virus was amplified and found to be 1858 nucleotides long, with an open reading frame of 1287 nucleotides that encodes a protein of 429 amino acids. The nucleotide sequence described here showed a high identity with the N protein gene of Araraquara virus. The entire N protein was expressed using the vector pET200D and the Escherichia coli BL21 strain. The expression of the recombinant protein was confirmed by the detection of a 52-kDa protein by Western blot using a pool of human sera obtained from HCPS patients, and by specific IgG detection in five serum samples of HCPS patients tested by ELISA. These results suggest that the recombinant N protein could be used as an antigen for the serological screening of hantavirus infection.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2008000700008
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.41 n.7 2008
Fonte: | info:eu-repo/semantics/openAccess