Data/hora: 04/06/2024 01:30:47

Provedor de dados: 	Brazilian Journal of Medical and Biological Research	
País: 	Brazil	
Título: 	A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus	
Autores: 	Cardoso,T.C.; Mouro-Sousa,R.L.; Oliveira,C.; Stringhini,G.; Augusto-Pinto,A.	
Data: 	1999-06-01	
Ano: 	1999	
Palavras-chave: 	Liquid phase blocking ELISA; Humoral response; Infectious bronchitis virus.	
Resumo: 	A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88%), specificity (100%) and agreement (95.31%).	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600010	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X1999000600010	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.32 n.6 1999	
Fonte: 	info:eu-repo/semantics/openAccess