Data/hora: 02/06/2024 06:53:29

Provedor de dados: | 56
País: | Brazil
Título: | An HPLC-UV method for the measurement of permeability of marker drugs in the Caco-2 cell assay
Autores: | Kratz,J.M.; Teixeira,M.R.; Koester,L.S.; Simões,C.M.O.
Data: | 2011-06-01
Ano: | 2011
Palavras-chave: | Caco-2; HPLC-UV; Marker drugs; Permeability.
Resumo: | The Caco-2 cell line has been used as a model to predict the in vitro permeability of the human intestinal barrier. The predictive potential of the assay relies on an appropriate in-house validation of the method. The objective of the present study was to develop a single HPLC-UV method for the identification and quantitation of marker drugs and to determine the suitability of the Caco-2 cell permeability assay. A simple chromatographic method was developed for the simultaneous determination of both passively (propranolol, carbamazepine, acyclovir, and hydrochlorothiazide) and actively transported drugs (vinblastine and verapamil). Separation was achieved on a C18 column with step-gradient elution (acetonitrile and aqueous solution of ammonium acetate, pH 3.0) at a flow rate of 1.0 mL/min and UV detection at 275 nm during the total run time of 35 min. The method was validated and found to be specific, linear, precise, and accurate. This chromatographic system can be readily used on a routine basis and its utilization can be extended to other permeability models. The results obtained in the Caco-2 bi-directional transport experiments confirmed the validity of the assay, given that high and low permeability profiles were identified, and P-glycoprotein functionality was established.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600006
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2011007500060
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.44 n.6 2011
Fonte: | info:eu-repo/semantics/openAccess