Data/hora: 01/06/2024 17:59:35

Provedor de dados: | 56
País: | Brazil
Título: | Efficacy of the dietary histone deacetylase inhibitor butyrate alone or in combination with vitamin A against proliferation of MCF-7 human breast cancer cells
Autores: | Andrade,F.O.; Nagamine,M.K.; Conti,A. De; Chaible,L.M.; Fontelles,C.C.; Jordão Junior,A.A.; Vannucchi,H.; Dagli,M.L.Z.; Bassoli,B.K.; Moreno,F.S.; Ong,T.P.
Data: | 2012-09-01
Ano: | 2012
Palavras-chave: | Breast cancer; MCF-7 cells; Butyric acid; Vitamin A; Epigenetics.
Resumo: | The combined treatment with histone deacetylase inhibitors (HDACi) and retinoids has been suggested as a potential epigenetic strategy for the control of cancer. In the present study, we investigated the effects of treatment with butyrate, a dietary HDACi, combined with vitamin A on MCF-7 human breast cancer cells. Cell proliferation was evaluated by the crystal violet staining method. MCF-7 cells were plated at 5 x 10(4) cells/mL and treated with butyrate (1 mM) alone or combined with vitamin A (10 µM) for 24 to 120 h. Cell proliferation inhibition was 34, 10 and 46% following treatment with butyrate, vitamin A and their combination, respectively, suggesting that vitamin A potentiated the inhibitory activities of butyrate. Furthermore, exposure to this short-chain fatty acid increased the level of histone H3K9 acetylation by 9.5-fold (Western blot), but not of H4K16, and increased the expression levels of p21WAF1 by 2.7-fold (Western blot) and of RARβ by 2.0-fold (quantitative real-time PCR). Our data show that RARβ may represent a molecular target for butyrate in breast cancer cells. Due to its effectiveness as a dietary HDACi, butyrate should be considered for use in combinatorial strategies with more active retinoids, especially in breast cancers in which RARβ is epigenetically altered.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2012007500103
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.45 n.9 2012
Fonte: | info:eu-repo/semantics/openAccess