Data/hora: 09/06/2024 10:30:04

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Detection of point mutations by non-isotopic single strand conformation polymorphism
Autores: | Pinheiro,N.A.; Moura,R.P.; Monteiro,E.; Villa,L.L.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | P53 gene; Human papillomavirus; PCR; SSCP; Cancer; Silver staining.
Resumo: | We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100008
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1999000100008
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.32 n.1 1999
Fonte: | info:eu-repo/semantics/openAccess