Data/hora: 28/05/2024 15:37:41

Provedor de dados: | 56
País: | Brazil
Título: | In vitro cytotoxicity of the LDE: daunorubicin complex in acute myelogenous leukemia blast cells
Autores: | Dorlhiac-Llacer,P.E.; Marquezini,M.V.; Toffoletto,O.; Carneiro,R.C.G.; Maranhão,R.C.; Chamone,D.A.F.
Data: | 2001-10-01
Ano: | 2001
Palavras-chave: | Cytotoxicity; Acute myelogenous leukemia; Daunorubicin; Low-density lipoprotein; LDE complex; RT-PCR.
Resumo: | Acute myelogenous leukemia (AML) blast cells show high-affinity degradation of low-density lipoprotein (LDL), suggesting an increased expression of cellular LDL receptors. LDE is a lipid microemulsion easily synthesized in vitro which is known to mimic the metabolic pathway of LDL. We used LDE as a carrier for daunorubicin and assayed the cytotoxicity of the complex using AML blast cells since RT-PCR analysis showed that AML cells express LDL receptor mRNA. The LDE:daunorubicin complex killed 46.7% of blast cells and 20.2% of normal bone marrow cells (P&lt;0.001; Student t-test). Moreover, this complex destroyed AML blast cells as efficiently as free daunorubicin. Thus, LDE might be a suitable carrier of chemotherapeutic agents targeting these drugs to neoplastic cells and protecting normal tissues.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000004
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2001001000004
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.34 n.10 2001
Fonte: | info:eu-repo/semantics/openAccess