Data/hora: 04/06/2024 01:44:11

Provedor de dados: | 56
País: | Brazil
Título: | Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals
Autores: | Barabás,É.; Nagy,K.; Várkonyi,V.; Horváth,A.
Data: | 2001-10-01
Ano: | 2001
Palavras-chave: | Quantitative competitive RT-PCR; Semiquantitative RT-PCR; IFN-gamma mRNA; HIV.
Resumo: | IFN-gamma mRNA expression was evaluated in nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT-PCR and the sensitivity of these methods was compared. A significant correlation was found between quantitative competitive and semiquantitative RT-PCR in samples of both HIV-seronegative (P = 0.004) and HIV-infected individuals (P = 0.0004). PBMC from HIV-infected individuals presented a remarkable increase of IFN-gamma mRNA expression, as determined by both types of RT-PCR methods. Semiquantitative RT-PCR even without an internal standard is also acceptable for measuring cytokine mRNA expression, but less reliable if small amounts are quantified. Moreover, we found that increased IFN-gammamRNA expression is independent of CD4+ cell count in AIDS-free HIV-infected patients.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000006
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2001001000006
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.34 n.10 2001
Fonte: | info:eu-repo/semantics/openAccess