Data/hora: 01/06/2024 17:51:11

Provedor de dados: | 56
País: | Brazil
Título: | Alterations in proteins of bone marrow extracellular matrix in undernourished mice
Autores: | Vituri,C.L.; Alvarez-Silva,M.; Trentin,A.G.; Borelli,P.
Data: | 2000-08-01
Ano: | 2000
Palavras-chave: | Fibronectin; Bone marrow; Extracellular matrix; Malnutrition.
Resumo: | The objective of the present study was to determine the effect of protein malnutrition on the glycoprotein content of bone marrow extracellular matrix (ECM). Two-month-old male Swiss mice were submitted to protein malnutrition with a low-protein diet containing 4% casein as compared to 20% casein in the control diet. When the experimental group had attained a 20% loss of their original body weight, we extracted the ECM proteins from bone marrow with PBS buffer, and analyzed ECM samples by SDS-PAGE (7.5%) and ECL Western blotting. Quantitative differences were observed between control and experimental groups. Bone marrow ECM from undernourished mice had greater amounts of extractable fibronectin (1.6-fold increase) and laminin (4.8-fold increase) when compared to the control group. These results suggest an association between fluctuations in the composition of the hematopoietic microenvironment and altered hematopoiesis observed in undernourished mice.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000800004
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2000000800004
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.33 n.8 2000
Fonte: | info:eu-repo/semantics/openAccess