Data/hora: 03/06/2024 18:01:32

Provedor de dados: | 56
País: | Brazil
Título: | Attenuation of phosphamidon-induced oxidative stress and immune dysfunction in rats treated with N-acetylcysteine
Autores: | Suke,S.G.; Ahmed,R.S.; Pathak,R.; Tripathi,A.K.; Banerjee,B.D.
Data: | 2008-09-01
Ano: | 2008
Palavras-chave: | Cytokines; Antioxidant; Organophosphate; Oxidative stress; Immunotoxicity.
Resumo: | The effect of N-acetylcysteine, a thiolic antioxidant, on attenuation of phosphamidon-induced oxidative stress and immune dysfunction was evaluated in adult male Wistar rats weighing 200-250 g. Rats were divided into four groups, 8 animals/group, and treated with phosphamidon, N-acetylcysteine or the combination of both for 28 days. Oral administration of phosphamidon (1.74 mg/kg), an organophosphate insecticide, increased serum malondialdehyde (3.83 ± 0.18 vs 2.91 ± 0.24 nmol/mL; P &lt; 0.05) and decreased erythrocyte superoxide dismutase (567.8 ± 24.36 vs 749.16 ± 102.61 U/gHb; P &lt; 0.05), catalase activity (1.86 ± 0.18 vs 2.43 ± 0.08 U/gHb; P &lt; 0.05) and whole blood glutathione levels (1.25 ± 0.21 vs 2.28 ± 0.08 mg/gHb; P &lt; 0.05) showing phosphamidon-induced oxidative stress. Phosphamidon exposure markedly suppressed humoral immune response as assessed by antibody titer to ovalbumin (4.71 ± 0.51 vs 8.00 ± 0.12 -log2; P &lt; 0.05), and cell-mediated immune response as assessed by leukocyte migration inhibition (25.24 ± 1.04 vs 70.8 ± 1.09%; P &lt; 0.05) and macrophage migration inhibition (20.38 ± 0.99 vs 67.16 ± 5.30%; P &lt; 0.05) response. Phosphamidon exposure decreased IFN-у levels (40.7 ± 3.21 vs 55.84 ± 3.02 pg/mL; P &lt; 0.05) suggesting a profound effect of phosphamidon on cell-mediated immune response. A phosphamidon-induced increase in TNF-α level (64.19 ± 6.0 vs 23.16 ± 4.0 pg/mL; P &lt; 0.05) suggests a contributory role of immunocytes in oxidative stress. Co-administration of N-acetylcysteine (3.5 mmol/kg, orally) with phosphamidon attenuated the adverse effects of phosphamidon. These findings suggest that oral N-acetylcysteine treatment exerts protective effect and attenuates free radical injury and immune dysfunction caused by subchronic phosphamidon exposure.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000900004
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2008000900004
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.41 n.9 2008
Fonte: | info:eu-repo/semantics/openAccess