Data/hora: 09/06/2024 06:06:08

Provedor de dados: | 56
País: | Brazil
Título: | Purification of bovine pancreatic glucagon as a by-product of insulin production
Autores: | Andrade,A.S.R.; Vilela,L.; Tunes,H.
Data: | 1997-12-01
Ano: | 1997
Palavras-chave: | Glucagon; Protein purification; Gel filtration; Ion-exchange chromatography; Insulin.
Resumo: | A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation. The isoelectric precipitate containing glucagon was then purified by ion-exchange chromatography on Q-Sepharose FF, gel filtration on Sephadex G-25 and ion-exchange chromatography on S-Sepharose FF. A pilot scale test was performed with a recovery of 87.6% and a purification factor of 8.78 for the first chromatographic step, a recovery of 75.1% and a purification factor of 3.90 for the second, and a recovery of 76.2% and a purification factor of 2.36 for the last one. The overall yield was 50%, a purification factor of 80.8 was obtained and the fraction containing active glucagon (suitable for pharmaceutical preparations) was 84% pure as analyzed by HPLC
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001200006
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X1997001200006
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.30 n.12 1997
Fonte: | info:eu-repo/semantics/openAccess