Data/hora: 11/06/2024 11:29:48

Provedor de dados: | 56
País: | Brazil
Título: | Liver cancer stem cells are selectively enriched by low-dose cisplatin
Autores: | Zhang,H.; Chang,W.J.; Li,X.Y.; Zhang,N.; Kong,J.J.; Wang,Y.F.
Data: | 2014-06-01
Ano: | 2014
Palavras-chave: | Cisplatin; Liver cancer stem cell; HepG2; ALDH1; CD133.
Resumo: | Accumulating evidence has indicated the importance of cancer stem cells in carcinogenesis. The goal of the present study was to determine the effect of low-dose cisplatin on enriched liver cancer stem cells (LCSCs). Human hepatoblastoma HepG2 cells were treated with concentrations of cisplatin ranging from 1 to 5 μg/mL. Cell survival and proliferation were evaluated using a tetrazolium dye (MTT) assay. LCSCs were identified using specific markers, namely aldehyde dehydrogenase-1 (ALDH1) and CD133. The percentage of ALDH1+ or CD133+ cells was examined by flow cytometric analysis. The expression of ALDH1 and/or CD133 in HepG2 cells was determined by immunocytochemical analysis. Low-dose cisplatin treatment significantly decreased cell survival in HepG2 cells after 24 or 72 h. However, the percentage of LCSCs in the surviving cells was greatly increased. The percentage of ALDH1+ or CD133+ cells was increased in a time- and dose-dependent manner after treatment with 1-4 μg/mL cisplatin, whereas 5 μg/mL cisplatin exposure slightly reduced the number of positive cells. These findings indicate that low-dose cisplatin treatment may efficiently enrich the LCSC population in HepG2 cells.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600478
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431X20143415
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.47 n.6 2014
Fonte: | info:eu-repo/semantics/openAccess