Data/hora: 17/06/2024 10:28:39

Provedor de dados: | Brazilian Journal of Medical and Biological Research
País: | Brazil
Título: | Inhibition of in vitro CO2 production and lipid synthesis by 2-hydroxybutyric acid in rat brain
Autores: | Silva,A.R.; Ruschel,C.; Helegda,C.; Wyse,A.T.S.; Wannmacher,C.M.D.; Wajner,M.; Dutra-Filho,C.S.
Data: | 2001-05-01
Ano: | 2001
Palavras-chave: | Lipid synthesis; Energy metabolism; Cerebral cortex; 2-hydroxybutyric acid.
Resumo: | 2-Hydroxybutyric acid appears at high concentrations in situations related to deficient energy metabolism (e.g., birth asphyxia) and also in inherited metabolic diseases affecting the central nervous system during neonatal development, such as "cerebral" lactic acidosis, glutaric aciduria type II, dihydrolipoyl dehydrogenase (E3) deficiency, and propionic acidemia. The present study was carried out to determine the effect of 2-hydroxybutyric acid at various concentrations (1-10 mM) on CO2 production and lipid synthesis from labeled substrates in cerebral cortex of 30-day-old Wistar rats in vitro. CO2 production was significantly inhibited (30-70%) by 2-hydroxybutyric acid in cerebral cortex prisms, in total homogenates and in the mitochondrial fraction. We also demonstrated a significant inhibition of lipid synthesis (20-45%) in cerebral cortex prisms and total homogenates in the presence of 2-hydroxybutyric acid. However, no inhibition of lipid synthesis occurred in homogenates free of nuclei and mitochondria. The results indicate an impairment of mitochondrial energy metabolism caused by 2-hydroxybutyric acid, a fact that may secondarily lead to reduction of lipid synthesis. It is possible that these findings may be associated with the neuropathophysiology of the situations where 2-hydroxybutyric acid is accumulated.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500010
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/S0100-879X2001000500010
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.34 n.5 2001
Fonte: | info:eu-repo/semantics/openAccess