Data/hora: 02/06/2024 12:39:23

Provedor de dados: | 56
País: | Brazil
Título: | Propofol inhibits lung cancer cell viability and induces cell apoptosis by upregulating microRNA-486 expression
Autores: | Yang,N.; Liang,Y.; Yang,P.; Yang,T.; Jiang,L.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Propofol; Lung cancer; Cell viability; Cell apoptosis; MicroRNA-486.
Resumo: | Propofol is a frequently used intravenous anesthetic agent. Recent studies show that propofol exerts a number of non-anesthetic effects. The present study aimed to investigate the effects of propofol on lung cancer cell lines H1299 and H1792 and functional role of microRNA (miR)-486 in these effects. H1299 and/or H1792 cells were treated with or without propofol and transfected or not with miR-486 inhibitor, and then cell viability and apoptosis were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry. The expression of miR-486 was determined by quantitative real-time polymerase chain reaction (qRT-PCR) with or without propofol treatment. Western blot was performed to analyze the protein expression of Forkhead box, class O (FOXO) 1 and 3, Bcl-2 interacting mediator of cell death (Bim), and pro- and activated caspases-3. Results showed that propofol significantly increased the miR-486 levels in both H1299 and H1792 cells compared to untreated cells in a dose-dependent manner (P&lt;0.05 or P&lt;0.01). Propofol statistically decreased cell viability but increased the percentages of apoptotic cells and protein expressions of FOXO1, FOXO3, Bim, and pro- and activated caspases-3; however, miR-486 inhibitor reversed the effects of propofol on cell viability, apoptosis, and protein expression (P&lt;0.05 or P&lt;0.01). In conclusion, propofol might be an ideal anesthetic for lung cancer surgery by effectively inhibiting lung cancer cell viability and inducing cell apoptosis. Modulation of miR-486 might contribute to the anti-tumor activity of propofol.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100601
Editor: | Associação Brasileira de Divulgação Científica
Relação: | 10.1590/1414-431x20165794
Formato: | text/html
Fonte: | Brazilian Journal of Medical and Biological Research  v.50 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess